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2 edition of Characterization of P-glycoprotein expression as a multixenobiotic resistance mechanism in fish found in the catalog.

Characterization of P-glycoprotein expression as a multixenobiotic resistance mechanism in fish

Shannon Mala Bard

Characterization of P-glycoprotein expression as a multixenobiotic resistance mechanism in fish

by Shannon Mala Bard

  • 282 Want to read
  • 31 Currently reading

Published by Massachusetts Institute of Technology, Woods Hole Oceanographic Institution in Cambridge, Mass, Woods Hole, Mass .
Written in English

    Subjects:
  • P-glycoprotein.,
  • Fishes -- Effect of water pollution on.,
  • Multidrug resistance.,
  • Xenobiotics -- Physiological effect.

  • Edition Notes

    Statementby Shannon Mala Bard.
    SeriesMIT/WHOI -- 2001-04., MIT/WHOI (Series) -- 2001-04.
    ContributionsWoods Hole Oceanographic Institution., Massachusetts Institute of Technology.
    The Physical Object
    Pagination258 p. :
    Number of Pages258
    ID Numbers
    Open LibraryOL17717911M

      PURPOSE To evaluate the prognostic significance of tumor cell P-glycoprotein (Pgp) expression at diagnosis in children with rhabdomyosarcoma. PATIENTS AND METHODS A panel of three anti-Pgp monoclonal antibodies (mAb) (C, C, and JSB-1) that recognize different Pgp epitopes was used to measure Pgp expression in rhabdomyosarcoma specimens obtained at Cited by:   To detect the expression of multidrug resistance molecules P-glycoprotein (P-gp), Lung resistnce protein (LRP) and Multidrug resistance-associated protein (MRP) and analyze the relationship between them and the clinico-pathological features. The expressions of P-gp, LRP and MRP in formalin-fixed paraffin-embedded tissue sections from 59 gastric cancer patients were determined by a Cited by:

    express P-glycoprotein as detected bymousemonoclonalanti-bodies against the humanmultidrug-resistance gene product. This pattern of endothelial cell expression may indicate a physiological role forP-glycoprotein in regulatingtheentryof certain molecules into the central nervous system and other anatomic compartments, such as the testes. These. P-glycoprotein was observed in 20 (65%) of the 31 HCCs. Cytoplasmic globular positivity was also seen in some cases. There were no significant associations betweenexpressionofP-glycoproteinand cell proliferation (determined by PCNA inimunoexpression and the mitotic count), or p53 expression. Patients with P-glycoprotein positive tumours had aCited by:

    We recently demonstrated that the coexpression of at least two proteins, including P glycoprotein, multidrug resistance-related protein, bcl-2 (flow cytometry), p53 (luminometric immunoassay), and heat shock protein 27 (Western blotting), was predictive for response to induction therapy in de novo AML comparing leukemic blasts of 20 responders with 20 by: @article{osti_, title = {Suppression of c-Myc is involved in multi-walled carbon nanotubes' down-regulation of ATP-binding cassette transporters in human colon adenocarcinoma cells}, author = {Wang, Zhaojing and Xu, Yonghong and Meng, Xiangning and Watari, Fumio and Liu, Hudan and Chen, Xiao}, abstractNote = {Over-expression of ATP-binding cassette (ABC) transporters, a large family of Author: Wang, Zhaojing.


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Characterization of P-glycoprotein expression as a multixenobiotic resistance mechanism in fish by Shannon Mala Bard Download PDF EPUB FB2

Request PDF | Characterization of the multixenobiotic resistance (MXR) mechanism in embryos and larvae of the zebra mussel (Dreissena polymorpha) and studies on its role in tolerance to single and.

Multidrug resistance (MDR) is a phenomenon by which tumor cells develop reduced sensitivity to anticancer drugs, which often leads to the failure of cancer chemotherapy.

A prominent mechanism of MDR is the overexpression of the multidrug efflux pump, P-glycoprotein (P-gp), which decreases the intracellular accumulation ofAuthor: Brian D. Lee. Abstract. P-glycoprotein, a transmembrane protein associated with multidrug resistance in cancer cells, is also expressed in normal tissues.

To get more insight into the physiologic role of mdr1/P-glycoprotein, we investigated its expression in human fetal tissues after 7 to 38 weeks of gestation by an immunohistochemical technique, using three different monoclonal antibodies, and by a Cited by: Expression, purification and mutagenesis of human P-glycoprotein in yeast.

To obtain large amounts of human P-glycoprotein (P-gp), we developed a plasmid-based, high-yield expression system in the yeast Saccharomyces sion was controlled either under the strong constitutive PMA1 promoter or the tightly regulated and inducible GAL1 by:   Multixenobiotic resistance (MXR) is an important mechanism of cellular efflux mediated by ATP binding cassette (ABC) transporters that bind and actively remove toxic substrates from the cell.

This study was the first to identify ABC transporter P-glycoprotein (P-gp/ABCB1) as a representative of the MXR phenotype in earthworm (Eisenia fetida).Cited by: Characterization of P-glycoprotein and multidrug resistance proteins in rat kidney and intestinal cell linesCited by: Effect of temperature on the expression of P-glycoprotein in the zebra mussel, Dreissena polymorpha Article in Journal of Thermal Biology 32(3) April with 32 Reads.

P‐Glycoprotein as a Hydrophobic Vacuum Cleaner or Drug Flippase. Role of the Lipid Bilayer in P‐Glycoprotein Function. Mechanism of Action of P‐Glycoprotein. Role of P‐Glycoprotein in Drug Therapy. Modulation of P‐Glycoprotein in Cancer Treatment. Regulation of P‐Glycoprotein ExpressionCited by: P-Glycoprotein.

P-glycoprotein (PGP) is an integral membrane transport protein that is encoded by the multidrug-resistance (MDR)-1 gene and functions as an energy-dependent efflux pump of metabolites and xenobiotic toxins from the intracellular space to the outside [].

P-glycoprotein is an important mediator of drug-drug interactions. 3 The pharmacokinetics of a drug may be altered when co-administered with compounds which inhibit or induce P-glycoprotein. 3, 5, 6 Inhibitors include clarithromycin, erythromycin, ritonavir and verapamil.

Inducers include rifampicin and St Cited by: P‐glycoprotein was phosphorylated by all of the tested PKC isoenzymes which included α, β‐I, β‐II, γ, δ, ε, ζ and η (Fig. These P‐glycoprotein‐expressing Sf9 cells contain very little endogenous PKC, which is a selective advantage for using this by: P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation (CD) is an important protein of the cell membrane that pumps many foreign substances out of cells.

More formally, it is an ATP-dependent efflux pump with broad substrate Aliases: ABCB1, ABC20, CD, CLCS, GP. Levels of multidrug resistance (MDR1) P-glycoprotein expression by human breast cancer correlate with in vitro resistance to taxol and doxorubicin.

Clinical Cancer Research 4, Cited by:   Canine hepatocellular carcinoma (HCC) arises from the uncontrolled proliferation of hepatocytes.

4, 5, 21 The causal mechanisms underlying this disease are not fully understood but are thought to involve environmental factors. Exposure to carcinogens or cancer-causing chemicals, including fungal toxins, food additives, pesticides, dyes, and plants, may increase the risk of cancer Cited by:   P-glycoprotein (Pgp), the product of the human MDR1 gene responsible for a major form of multidrug resistance in tumor cells, is a transmembrane protein that carries out ATP-dependent efflux of various lipophilic compounds, including many anticancer drugs.

The relationship between P-glycoprotein expression and malignancy is controversial. We have recently found that, in osteosarcoma, multidrug resistance (MDR) is associated with a Cited by:   It is not known if P-glycoprotein alone or P-glycoprotein together with another drug-resistance mechanism contributes to the failure of chemotherapy.

The clinical significance of other such proteins, including the multidrug resistance protein, lung resistance protein, glutathione enzymes, or topoisomerase II, is poorly defined at present for Cited by:   To clarify the function of the multidrug transporter P-glycoprotein in mast cells we used the green fluorescent compound Bodipy-FL-verapamil, which is a substrate of P-glycoprotein.

This compound is also transported by Multidrug Resistance-related Protein (MRP), another membrane transport protein expressed in many tumour resistant cells as well as in normal by: 5.

The MDR1 gene‐product P‐glycoprotein (P‐gp/ABCB1), an ATP‐dependent efflux pump, plays an essential role in the efflux transport of hydrophobic xenobiotics and peptides from the inside to the outside of cells. 1, 2 P‐gp is highly expressed in the plasma membrane in several tumor cells as a mechanism responsible for resistance against Cited by:   Figure 1.

High ABCB1 mRNA is associated with resistance to microtubule-targeting agents in cell lines.A, P-gp expression strongly associates with lack of response to vinblastine (P = ) and paclitaxel (P = 1E−29) in a panel of N = solid cancer cell lines ().Gene expression levels are color-coded from red (high expression) to green (low expression), with black indicating mean by: 7.

P-glycoprotein is a member of a class of transport molecules called "ATP Binding Cassette" transporters or "ABC" transporters for short Illustration of p-glycoprotein activity Intestines - p-glycoprotein limits drug absorption from the intestine.

The purpose of this study was to retrospectively study 48 patients with infiltrating ductal breast cancer to evaluate the relationship between the degree of accumulation of technetiumm methoxyisobutylisonitrile (Tc-MIBI) and P-glycoprotein (Pgp) or multidrug resistance-related protein (MRP) expression in breast cancer tissues.

Before surgery or biopsy, all 48 patients underwent Cited by: A similar phenomenon has also been observed and considered as an important part of the multixenobiotic resistance (MXR) defence system in aquatic organisms. We have recently demonstrated the presence of ABC transporters in the widely used in vitro fish model, the PLHC-1 hepatoma cell line.